Oligonucleotide probes for detection of cephalosporinases among Bacteroides strains
نویسندگان
چکیده
منابع مشابه
Characterization of cephalosporinases from Bacteroides fragilis, Bacteroides thetaiotaomicron and Bacteroides vulgatus.
The susceptibility of 80 Bacteroides fragilis group strains isolated from clinical specimens to beta-lactam antibiotics was investigated by agar dilution method. Twenty strains showed high resistance to the antibiotics. The resistance level of the isolates to cephaloridine was related to the amount of beta-lactamase activity (cephalosporinase; CSase) produced. B. fragilis GN-11477, B. thetaiota...
متن کاملEvaluation of Fluoretec-M for detection of oral strains of Bacteroides asaccharolyticus and Bacteroides melaninogenicus.
Fluoretec-M is a polyvalent conjugate used in direct fluorescent-antibody staining for identification of the Bacteroides asaccharolyticus-Bacteroides melaninogenicus group. The Fluoretec-M reagent detected all oral and nonoral test strains of B. melaninogaenicus subsp. intermedius, all test strains of B. melaninogenicus subsp. melaninogenicus, and the nonoral strains of B. asaccharolyticus. Ho...
متن کاملMicroarray oligonucleotide probes.
Oligonucleotide probes are increasingly the method of choice for many modern DNA microarray applications. They provide higher target specificity, probe selection gives improved experimental control of hybridization properties, and targeting of specific gene subsequences allows better discrimination of highly similar targets such as splice variants or gene families. Only recently has there been ...
متن کاملFacile SNP detection using bifunctional, cross-linking oligonucleotide probes
A facile, sensitive method for detecting specific sequences of oligonucleotides was developed. Detection of DNA sequences with single nucleotide discrimination is achieved by combining the selectivity of hybridization with an efficient cross-linking reaction. Readily synthesized bifunctional oligonucleotide probes containing a modified pyrimidine that is capable of forming interstrand cross-lin...
متن کاملROSO: optimizing oligonucleotide probes for microarrays
UNLABELLED ROSO is software to design optimal oligonucleotide probe sets for microarrays. Selected probes show no significant cross-hybridization, no stable secondary structures and their Tm are chosen to minimize the Tm variability of the probe set. AVAILABILITY The program is available on the internet. Sources are freely available, for non-profit use, on request to the authors. SUPPLEMENT...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1996
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.40.4.1014